Cell Staining: Fluorescent Labelling of Endocytic Compartments
Jean‐Pierre Gorvel, INSERM‐CNRS de Marseille‐Luminy, Marseille, France
Stéphane Méresse, INSERM‐CNRS de Marseille‐Luminy, Marseille, France
Published online: April 2001
DOI: 10.1038/npg.els.0002630
Abstract
Endocytic compartments are acidic organelles involved in the transport of extracellular solutes and ligands from the plasma
membrane to early, then to late endosomes and eventually to lysosomes. Endocytic compartments can be fluorescently labelled
by fluid phase endocytosis, internalized specific ligands, resident proteins and pHâsensitive probes.
Keywords: endocytosis; endosome; lysosome; membrane traffic; green fluorescent protein
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