Cell Staining: Fluorescence imaging of mitochondrial structure and function

Robert H Pierce, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Terrance J Kavanagh, University of Washington, Seattle, Washington, USA
Martin Poot, University of Washington, Seattle, Washington, USA

Published online: April 2001

DOI: 10.1038/npg.els.0002632

Staining of mitochondria with fluorescent dyes, antibodies or mitochondria-targeted naturally fluorescent molecules contributes to the study of mitochondrial structure and function in normal physiological and pathophysiological states. Using these techniques, the mitochondrial permeability transition and redox state can be probed.

Keywords: cell staining; fluorescent labelling; permeability transition; membrane potential; apoptosis; reactive oxygen species

Figure 1. Mouse hepatocytes stained with FITC-conjugated anticytochrome C antibody (green) and nuclei stained with DAPI (blue).
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Cell Compartments and Cellular Organelles | Techniques in Cell Biology
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Article Title: Cell Staining: Fluorescence imaging of mitochondrial structure and function
 
How to Cite close
Pierce, Robert H, Kavanagh, Terrance J, and Poot, Martin(Apr 2001) Cell Staining: Fluorescence imaging of mitochondrial structure and function. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002632]