Semliki Forest Virus Expression System

Mathilda Sjöberg, Karolinska Institute, Huddinge, Sweden
Maria Ekström, Karolinska Institute, Huddinge, Sweden

Published online: September 2005

DOI: 10.1038/npg.els.0003860

Eukaryotic cells can be programmed to synthesize foreign proteins by infection with recombinant Semliki Forest virus. The foreign protein is produced at a high level from about 4 h after infection, whereas cell-derived protein synthesis is shut down.

Keywords: mammalian protein expression; biotechnology

 References
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 Further Reading
    book Ekström M, Garoff H and Andersson H (1998) "Semliki Forest virus expression system". In: Celis JE (ed.) Cell Biology: A Laboratory Handbook, pp. 218–224. San Diego, CA: Academic Press.
    Garoff H and Li K-J (1998) Recent advances in gene expression using alphavirus vectors. Current Opinion in Biotechnology 9: 464–469.
    book Liljeström P and Garoff H (1995) "Expression of proteins using Semliki Forest virus vectors". In: Chanda VB (ed.) Current Protocols in Molecular Biology, pp. 16.20.01–16.20.13. Chichester, UK: Wiley.
    Strauss JH and Strauss EG (1994) The alpha viruses: gene expression, replication and evolution. Microbiological Reviews 58: 491–562.

Related Sub-Topics:

RNA Viruses | Virus Replication | Translation and Protein synthesis | Virus Structure and Function | Sample Preparation in Structural Biology | Single Molecule Analysis | Techniques and Tools in Molecular Biology | Nonchromosomal DNA
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Article Title: Semliki Forest Virus Expression System
 
How to Cite close
Sjöberg, Mathilda, and Ekström, Maria(Sep 2005) Semliki Forest Virus Expression System. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003860]