Gene Transfer and Expression in Tissue Culture Cells

Abstract

Gene transfer and expression in cultured mammalian cells comprise a complex of techniques to introduce and express novel genetic material in a variety of eukaryotic cell types in an effort to examine the possible effect of its activity on intracellular processes.

Keywords: gene transfer; transfection; cDNA; expression vector

Figure 1.

Methods for gene transfer into mammalian cells.

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References

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Further Reading

Ravid K and Freshney I (eds) (1998) DNA Transfer to Cultured Cells. New York: Wiley‐Liss.

Hauser H and Wagner R (eds) (1997) Mammalian Cell Biotechnology in Protein Production. Berlin: Walter de Gruyter.

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How to Cite close
Gromov, Pavel S, and Celis, Julio E(Apr 2001) Gene Transfer and Expression in Tissue Culture Cells. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002662]