Miguel L Allende, University of Chile, Santiago, Chile
Published online: April 2001
DOI: 10.1038/npg.els.0002670
Gene transfer can be readily achieved in zebrafish by microinjection of plasmid DNA or retroviral infection in early embryos. Stable integration of plasmid DNA allows efficient expression of foreign genes either ubiquitously or tissue-specifically, depending on the type of promoter used. Most zebrafish transgenics generated to date have been used in basic embryological research.
Keywords: gene transfer; mutagenesis; danio rerio; microinjection
| References | |
| Amsterdam A, Lin S and Hopkins N (1995) The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Developmental Biology 171: 123–129. | |
| Caldovic L and Hackett P (1995) Development of position-independent expression vectors and their transfer into transgenic fish. Molecular Marine Biology and Biotechnology 4: 51–61. | |
| Collas P and Alestrom P (1998) Nuclear localization signals enhance germline transmission of a transgene in zebrafish. Transgenic Research 7: 303–309. | |
| Culp P, Nüsslein-Volhard C and Hopkins N (1991) High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proceedings of the National Academy of Sciences of the USA 88: 7953–7957. | |
| Gaiano N, Amsterdam A, Kawakami K, Allende M, Becker T and Hopkins N (1996) Insertional mutagenesis and rapid cloning of essential genes in zebrafish. Nature 383: 829–832. | |
| Higashijima S, Okamoto H, Ueno N, Hotta Y and Eguchi G (1997) High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin. Developmental Biology 192: 289–299. | |
| Ivics Z, Hackett P, Plasterk R and Izsvak Z (1997) Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 91: 501–510. | |
| proceedings Jessen J, Meng A, McFarlane R et al. (1998) Modification of bacterial artificial chromosomes through Chi-stimulated homologous recombination and its application in zebrafish transgenesis. Proceedings of the National Academy of Sciences of the USA 95: 5121–5126. | |
| Lin S, Gaiano N, Culp P et al. (1994a) Integration and germ-line transmission of a pseudotyped retroviral vector in zebrafish. Science 265: 666–669. | |
| Lin S, Yang S and Hopkins N (1994b) lacZ expression in germline transgenic zebrafish can be detected in living embryos. Developmental Biology 161: 77–83. | |
| Long Q, Meng A, Wang H et al. (1997) GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development 124: 4105–4111. | |
| Raz E, van Luenen H, Schaerringer B, Plasterk R and Driever W (1998) Transposition of the nematode Caenorhabditis elegans Tc3 element in the zebrafish Danio rerio. Current Biology 8: 82–88. | |
| Stuart G, McMurray J and Westerfield M (1988) Replication, integration, and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development 103: 403–412. | |
| Yan Y, Talbot W, Egan E and Postlethwait J (1998) Mutant rescue by BAC clone injection in zebrafish. Genomics 50: 287–289. | |
| Further Reading | |
| Gaiano N and Hopkins N (1996) Introducing genes into zebrafish. Biochimica et Biophysica Acta 1288: O11–O14. | |
| Houdebine L and Chourrout D (1991) Transgenesis in fish. Experientia 47: 891–897. | |
| Iyengar A, Muller F and Maclean N (1996) Regulation and expression of transgenes in fish – a review. Transgenic Research 5: 147–166. | |
| book Meng A, Jessen J and Lin S (1999) "Transgenics". In: Detrich III HW, Westerfield M and Zon L (eds) "The Zebrafish: Genetics and Genomics". Methods in Cell Biology, vol. 60, pp. 133–148. San Diego: Academic Press. | |
| book Westerfield M (ed.) (1993) The Zebrafish Book. Eugene: University of Oregon Press. | |
Copyright © 2000-2013 by John Wiley & Sons, Inc., or related companies. All rights reserved.
