Miguel L Allende, University of Chile, Santiago, Chile
Published online: April 2001
DOI: 10.1038/npg.els.0002670
Gene transfer can be readily achieved in zebrafish by microinjection of plasmid DNA or retroviral infection in early embryos. Stable integration of plasmid DNA allows efficient expression of foreign genes either ubiquitously or tissueâspecifically, depending on the type of promoter used. Most zebrafish transgenics generated to date have been used in basic embryological research.
Keywords: gene transfer; mutagenesis; danio rerio; microinjection
References
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Further Reading
Gaiano N and Hopkins N (1996) Introducing genes into zebrafish. Biochimica et Biophysica Acta 1288: O11–O14.
Houdebine L and Chourrout D (1991) Transgenesis in fish. Experientia 47: 891–897.
Iyengar A, Muller F and Maclean N (1996) Regulation and expression of transgenes in fish – a review. Transgenic Research 5: 147–166.
Meng A, Jessen J and Lin S (1999) Transgenics. In: Detrich III HW, Westerfield M and Zon L (eds) The Zebrafish: Genetics and Genomics. Methods in Cell Biology, vol. 60, pp. 133–148. San Diego: Academic Press.
Westerfield M (ed.) (1993) The Zebrafish Book. Eugene: University of Oregon Press.
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