Protein Determination

Abstract

Protein determination aims at estimating the concentration of protein by comparing the unknown sample with a known standard. Sample preparation and choice of a suitable standard are the most important criteria for the accuracy of the results.

Keywords: Bradford assay; dot‐blot assay; interference; Lowry assay; protein determination

References

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Guttenberger M (2004) Protein determination. In: Celis JE (ed.) Cell Biology. A Laboratory Handbook, San Diego: Academic Press (in press).

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Guttenberger M, Schaeffer C and Hampp R (1994) Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce (Picea abies [L.] Karst.) employing a rapid one‐step extraction procedure. Trees 8: 191–197.

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Further Reading

Hjelmeland LM and Chrambach A (1984) Solubilization of functional membrane proteins. Methods in Enzymology 104: 305–318.

Kirazov LP, Venkov LG and Kirazov EP (1993) Comparison of the Lowry and the Bradford protein assays as applied for protein estimation of membrane‐containing fractions. Analytical Biochemistry 208: 44–48.

Marshall T and Williams KM (1992) Coomassie blue protein dye‐binding assays measure formation of an insoluble protein–dye complex. Analytical Biochemistry 204: 107–109.

Neuhoff V, Philipp K, Zimmer H‐G and Mesecke S (1979) A simple, versatile, sensitive and volume‐independent method for quantitative protein determination which is independent of other external influences. Hoppe‐Seyler's Zeitschrift für Physiologische Chemie 360: 1657–1670.

Neuhoff V, Ewers E and Huether G (1981) Spot analysis for glycoprotein determination in the nanogram range. Hoppe‐Seyler's Zeitschrift für Physiologischen Chemie 362: 1427: 1434.

Shakir FK, Audilet D, Drake AJ III and Shakir KMM (1994) A rapid protein determination by modification of the Lowry procedure. Analytical Biochemistry 216: 232: 233.

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How to Cite close
Guttenberger, Martin(May 2005) Protein Determination. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003916]