Peptide Mapping

Ralph C Judd, University of Montana, Missoula, Montana, USA

Published online: April 2001

DOI: 10.1038/npg.els.0002675

Peptide mapping is a technique for comparing protein primary structures and determining surface-exposed sites or epitopes within proteins. It can be adapted to obtain internal protein sequences.

Keywords: peptide; separation; SDS-PAGE; HPLC; sequencing

 References
    Judd RC (1983) 125I-labeled peptide mapping and high-performance liquid chromatography 125I-peptide separation of protein I of four strains of Neisseria gonorrhoeae. Journal of Liquid Chromatography 6: 1421–1439.
    Judd RC (1986) Evidence for N-terminal exposure of the PIA subclass of protein I of Neisseria gonorrhoeae. Infection and Immunity 54: 408–414.
    Judd RC (1987) Radioiodination and 125I-labeled peptide mapping on nitrocellulose membranes. Analytical Biochemistry 160: 306–315.
    book Judd RC (1990) "Peptide mapping". In: Deutscher MP (ed.) Methods in Enzymology: A Guide to Protein Purification, pp. 613–625. Orlando: Academic Press.
    book Judd RC (1994) "Electrophoresis of peptides". In: Walker JM (ed.) Basic Protein and Peptide Protocols, Methods in Molecular Biology, vol. 32, pp. 49–58. Totowa: Humana Press.
    Judd RC and Caldwell HD (1985a) Comparison of 125I- and 14C-radio-labeled peptides of the major outer membrane protein of Chlamydia trachomatis strain L2/434 separated by high-performance liquid chromatography. Journal of Liquid Chromatography 8: 1109–1120.
    Judd RC and Caldwell HD (1985b) Identification and isolation of surface-exposed portions of the major outer membrane protein of Chlamydia trachomatis by two-dimensional peptide mapping and high-performance liquid chromatography. Journal of Liquid Chromatography 8: 1559–1571.
    Porcella SF, Belland RJ and Judd RC (1996) Identification of an EF-Tu protein that is periplasm-associated and processed in Neisseria gonorrhoeae. Microbiology 142: 2481–2489.
 Further Reading
    book Judd RC (1996) "SDS-polyacrylamide gel electrophoretic separation of small peptides". In: Walker JM (ed.). The Protein Protocols Handbook, pp. 101–108. Totowa, NJ: Humana Press.
    book Judd RC (1996) "Peptide mapping by two-dimensional thin-layer electrophoresis thin-layer chromatography". In: Walker JM (ed.) The Protein Protocols Handbook, pp. 437–446. Totowa, NJ: Humana Press.
    book Judd RC (1996) "Peptide mapping by sodium dodecyl sulfate-polyacrylamide gel electrophoresis". In: Walker JM (ed.) The Protein Protocols Handbook, pp. 447–451. Totowa, NJ: Humana Press.
    book Judd RC (1996) "Peptide mapping by high-performance liquid chromatography". In: Walker JM (ed.) The Protein Protocols Handbook, pp. 453–455. Totowa, NJ: Humana Press.

Related Sub-Topics:

Protein Structure | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism
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Article Title: Peptide Mapping
 
How to Cite close
Judd, Ralph C(Apr 2001) Peptide Mapping. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002675]