Mohammad Azam Mansoor, Agder University College, Kristiansand, Norway
Published online: September 2005
DOI: 10.1038/npg.els.0003917
Liquid chromatography, also called high-performance liquid chromatography, is a popular quantitative analytical technique applied in many areas of chemical, biomedical and pharmaceutical sciences. Liquid chromatography currently accounts for about 60% of separation technology applied in life sciences around the world.
Keywords: HPLC; analysis; solutes; solvents; adsorbents; counter-ions
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Figure 1. A typical HPLC system consisting of a degasser, pump, mobile-phase delivery system (isocratic or gradient) detector (UV, fluorescence or electrochemical), autosampler and integrator (computer-aided). The column may be placed in an oven for constant temperature.
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Figure 2. A typical chromatogram of a monobromobimane (mBrB)-derivatized plasma sample of (a) a healthy control subject; (b) an epilepsy patient on anti epileptic drug, (c) a patient with kidney disease. Samples were analysed according to the method of Mansoor et al. (1992). Cys, cysteine, CG, cysteinylglycine; HCY, homocysteine. Column, mm×mm i.d.; stationary phase, particles ODS 3; mobile phase contains ion-pairing agent tetrabutylammonium phosphate; column temperature 25°C.
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| Further Reading | |
| Cremonesi L, Stenirri S, Fermo I et al. (2003) Denaturing HPLC analysis of DNA deletions and insertions. Human Mutation 22: 98–102. | |
| Dolan JW (2000a) Starting out right, Part I. Selecting the tools. LC-GC 13: 12–15 [www.lcgcmag.com]. | |
| Dolan JW (2000b) Starting out right, Part II. Measuring satisfaction. LC-GC 13: 72–76 [www.lcgcmag.com]. | |
| Dorsey JG, Foley JP, Cooper WT, Barford RA and Barth HG (1990) Liquid chromatography: theory and methodology. Analytical Chemistry 62: 324R–356R. | |
| Frueh FW and Noyer-Weidner M (2003) The use of denaturing high-performance liquid chromatography (DHPLC) for the analysis of genetic variations: impact for diagnostics and pharmacogenetics. Clinical Chemistry and Laboratory Medicine 41: 452–461. | |
| Kwok PY and Chen X (2003) Detection of single nucleotide polymorphisms. Current Issues in Molecular Biology 5: 43–60. | |
| LaBerge GS, Shelton RJ and Danielson PB (2003) Forensic utility of mitochondrial DNA analysis based on denaturing high-performance liquid chromatography. Croatian Medical Journal 44: 281–288. | |
| book Lim CK (1986) HPLC of Small Molecules, a Practical Approach. Oxford: IRL Press. | |
| book Lough WJ and Wainer IW (eds) (1996) High Performance Liquid Chromatography, Fundamental Principles and Practice. London: Blackie Academic and Professional. | |
| Mansoor MA, Svardal AM and Ueland PM (1992) Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine and glutathione in human plasma. Analytical Biochemistry 200: 218–229. | |
| Premstaller A and Oefner PJ (2003) Denaturing HPLC of nucleic acids. PharmaGenomics 3: 20–37. | |
| Snyder LR (2000) HPLC: past and present. Analytical Biochemistry 72: 412A–420A. | |
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