Figure 1. Representative protein patterns in SDS-PAGE (a) and PAGE under native conditions (b). The identical protein exhibits a single band after reaction with SDS (a) and four components of identical size and different net charge under native conditions (b). Left lane of (a) shows molecular weight markers. From Radko SP et al. (1999) Analytical Biochemistry 274: 146–148 (with permission of Academic Press).

Figure 2. Protein components of -amyloid. Gel pattern (a) and Ferguson plots (b). The slope (K_R) and intercept (Y₀) of these plots provide the size and net charge, respectively, of each of the four species. From Brining S et al. (1999) Electrophoresis 20: 1398–1402 (with permission of Wiley-VCH). %T designates total monomer concentration.

Figure 3. Confidence envelopes for the data of Figure 2. Horizontal alignment of these envelopes reveals aggregation states of the protein; vertical alignment reveals charge modification of a single-sized protein.