Gel Electrophoresis: One‐dimensional


Gel electrophoresis in a single direction of migration is termed ‘one‐dimensional’. It is presently the most widely used mode of gel electrophoretic separation.

Keywords: electrophoresis; gel; band identity; molecular size

Figure 1.

Representative protein patterns in SDS‐PAGE (a) and PAGE under native conditions (b). The identical protein exhibits a single band after reaction with SDS (a) and four components of identical size and different net charge under native conditions (b). Left lane of (a) shows molecular weight markers. From Radko SP et al. (1999) Analytical Biochemistry274: 146–148 (with permission of Academic Press).

Figure 2.

Protein components of β‐amyloid. Gel pattern (a) and Ferguson plots (b). The slope (KR) and intercept (Y0) of these plots provide the size and net charge, respectively, of each of the four species. From Brining S et al. (1999) Electrophoresis20: 1398–1402 (with permission of Wiley‐VCH). %T designates total monomer concentration.

Figure 3.

Confidence envelopes for the data of Figure 2. Horizontal alignment of these envelopes reveals aggregation states of the protein; vertical alignment reveals charge modification of a single‐sized protein.


Further Reading

Andrews AT (1986) Electrophoresis, 2nd edn. Oxford: Clarendon Press.

Chrambach A (1985) The Practice of Quantitative Gel Electrophoresis. Weinheim: VCH.

Chrambach A (1996) Quantitative and automated electrophoresis in sieving media: past, present and future. Electrophoresis 17: 454–464.

Rodbard D and Chrambach A (1974) Quantitative polyacrylamide gel electrophoresis (PAGE): mathematical and statistical analysis of PAGE data. In: Allen RC and Maurer HR (eds) Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel, pp. 28–62. Berlin: Walter de Gruyter.

Westermeier R (1997) Electrophoresis in Practice, 2nd edn. Weinheim: VCH.

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Chrambach, Andreas(Apr 2001) Gel Electrophoresis: One‐dimensional. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002683]