Autoradiography and Fluorography


Radioactive compounds are used to detect and monitor labelled cellular components by imaging on photographic emulsion in contact with the sample. In direct autoradiography, emissions from high‐energy radioisotopes directly affect the emulsion. In indirect autoradiography, a fluorescent intensifying screen produces photons that affect the emulsion; and in fluorography the emulsion detects photons produced when radioactive emissions interact with an added scintillant and the techique can use low‐energy emitters.

Keywords: radioactive isotopes; X‐ray film; radioactivity; intensifying screen; phosphorimager


Englert D, Roessler N, Jeavons A and Fairless S (1995) Microchannel array detector for quantitative electronic radioautography. Cellular and Molecular Biology 41: 57–64.

Gromov PS and Celis JE (1997) Two‐dimensional analysis of posttranslationally modified proteins. In: Cells JE, Carter N, Hunter T, et al. (eds) Cell Biology, A Laboratory Handbook, vol. 4, pp. 409–417. San Diego: Academic Press.

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Roberts PL (1985) Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters. Analytical Biochemistry 147: 521–524.

Suissa M (1983) Spectrophotometric quantitation of silver grains eluted from autoradiograms. Analytical Biochemistry 133: 511–514.

Further Reading

Johnston RJ, Pickett SC and Barker DL (1990) Autoradiography using storage phosphor technology. Electrophoresis 11: 355–360.

Wegmann R, Balmain N, Richard‐Blum S and Guha S (1995) Radioautography in cellular and molecular biology. Cellular and Molecular Biology (Noisy‐le‐grand) 41: 1–20.

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How to Cite close
Gromov, Pavel S, and Celis, Julio E(Mar 2003) Autoradiography and Fluorography. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002685]