Autoradiography and Fluorography

Abstract

Radioactive compounds are used to detect and monitor labelled cellular components by imaging on photographic emulsion in contact with the sample. In direct autoradiography, emissions from high‐energy radioisotopes directly affect the emulsion. In indirect autoradiography, a fluorescent intensifying screen produces photons that affect the emulsion; and in fluorography the emulsion detects photons produced when radioactive emissions interact with an added scintillant and the techique can use low‐energy emitters.

Keywords: radioactive isotopes; X‐ray film; radioactivity; intensifying screen; phosphorimager

References

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Gromov PS and Celis JE (1997) Two‐dimensional analysis of posttranslationally modified proteins. In: Cells JE, Carter N, Hunter T, et al. (eds) Cell Biology, A Laboratory Handbook, vol. 4, pp. 409–417. San Diego: Academic Press.

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Further Reading

Johnston RJ, Pickett SC and Barker DL (1990) Autoradiography using storage phosphor technology. Electrophoresis 11: 355–360.

Wegmann R, Balmain N, Richard‐Blum S and Guha S (1995) Radioautography in cellular and molecular biology. Cellular and Molecular Biology (Noisy‐le‐grand) 41: 1–20.

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How to Cite close
Gromov, Pavel S, and Celis, Julio E(Mar 2003) Autoradiography and Fluorography. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002685]