Autoradiography and Fluorography

Pavel S Gromov, University of Aarhus, Aarhus, Denmark
Julio E Celis, University of Aarhus, Aarhus, Denmark

Published online: March 2003

DOI: 10.1038/npg.els.0002685

Radioactive compounds are used to detect and monitor labelled cellular components by imaging on photographic emulsion in contact with the sample. In direct autoradiography, emissions from high-energy radioisotopes directly affect the emulsion. In indirect autoradiography, a fluorescent intensifying screen produces photons that affect the emulsion; and in fluorography the emulsion detects photons produced when radioactive emissions interact with an added scintillant and the techique can use low-energy emitters.

Keywords: radioactive isotopes; X-ray film; radioactivity; intensifying screen; phosphorimager

 References
    Englert D, Roessler N, Jeavons A and Fairless S (1995) Microchannel array detector for quantitative electronic radioautography. Cellular and Molecular Biology 41: 57–64.
    book Gromov PS and Celis JE (1997) "Two-dimensional analysis of posttranslationally modified proteins". In: Cells JE, Carter N, Hunter T, et al. (eds) Cell Biology, A Laboratory Handbook, vol. 4, pp. 409–417. San Diego: Academic Press.
    Quemeneur E and Simonnet F (1995) Increased sensitivity of autoradiography and fluorography by membrane blotting. Biotechniques 100: 102–103.
    Roberts PL (1985) Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters. Analytical Biochemistry 147: 521–524.
    Suissa M (1983) Spectrophotometric quantitation of silver grains eluted from autoradiograms. Analytical Biochemistry 133: 511–514.
 Further Reading
    Johnston RJ, Pickett SC and Barker DL (1990) Autoradiography using storage phosphor technology. Electrophoresis 11: 355–360.
    Wegmann R, Balmain N, Richard-Blum S and Guha S (1995) Radioautography in cellular and molecular biology. Cellular and Molecular Biology (Noisy-le-grand) 41: 1–20.

Related Sub-Topics:

Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Autoradiography and Fluorography
 
How to Cite close
Gromov, Pavel S, and Celis, Julio E(Mar 2003) Autoradiography and Fluorography. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002685]