Electroelution of Proteins from Polyacrylamide Gels

Abstract

Electroelution of proteins from gels is the method used to recover proteins resolved by electrophoresis in polyacrylamide gel by transferring the protein molecules out of gel by means of an electric field.

Keywords: gel electrophoresis; polyacrylamide gel slices; electroelution

Figure 1.

Schematic diagram of gel‐elution procedure (model 422 Electro‐Eluter, Bio‐Rad). Eluted proteins are collected in the volume between the porous filter and the dialysis membrane.

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References

Celis JE, Ratz G and Basse B (1997) Electroelution of proteins from two‐dimensional gels. In: Celis JE, Carter NP, Hunter T et al. (eds) Cell Biology. A Laboratory Handbook, vol. 4, pp. 404–407. New York: Academic Press.

Gulle H, Kaufmann SHE and Moriarty KM (1993) Rapid electroelution of two‐dimensionally separated protein mixtures: Its use in in vitro assays of T cell activities. Electrophoresis 14(9): 902–908.

Hall SC, Smith DM, Masiarz FR et al. (1993) Mass spectrometric and Edman sequencing of lipocortin I isolated by two‐dimensional SDS/PAGE of human melanoma lysates. Proceedings of the National Academy of Sciences of the USA 90(5): 1927–1931.

Henderson LE, Oroszlan S and Konigsberg W (1979) A micromethod for complete removal of dodecyl sulfate from proteins by ion‐pair extraction. Analytical Biochemistry 93(1): 153–157.

Liang S‐P, Lee TT and Laursen RA (1991) Single‐step electroelution of proteins from SDS‐polyacrylamide gels and immobilization on diisothiocyanate‐glass beads in prepacked capillary columns for solid‐phase microsequencing. Analytical Biochemistry 197(1): 163–167.

Schuhmacher M, Glocker M, Wunderlin M and Przybylski M (1996) Direct isolation of proteins from sodium dodecyl sulfate–polyacrylamide gel electrophoresis and analysis by electrospray‐ionization mass spectrometry. Electrophoresis 17(5): 848–854.

Further Reading

Dunn MJ (1996) Electroelution of proteins from polyacrylamide gels. In: Doonan S (ed.) Methods in Molecular Biology. Protein Purification Protocols, vol. 59, pp. 357–362. Totowa, NJ: Humana Press.

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How to Cite close
Gromov, Pavel S, and Celis, Julio E(Oct 2002) Electroelution of Proteins from Polyacrylamide Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002687]