Edman chemistry is a long-established approach to determination of the primary structure, or sequence, of polypeptides.
Keywords: Edman chemistry; peptide sequence; protein sequence
Peptide Sequencing by Edman Degradation
John Bryan Smith, Celltech Chiroscience plc, Slough, UK
Published online: April 2001
DOI: 10.1038/npg.els.0002688
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Figure 1. Edman chemistry for N-terminal sequencing of polypeptides.
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| References | |
| Edman P (1950) Method for determination of the amino acid sequence in peptides. Acta Chimica Scandinavica 4: 283–293. | |
| Edman P and Begg G (1967) A protein sequenator. European Journal of Biochemistry 1: 80–91. | |
| Hewick RM, Hunkapillar MW, Hood LE and Dreyer WJ (1981) A gas–liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry 256: 7990–7997. | |
| book Hirano H, Komatsu S and Tsunasawa S (1977) "On membrane deblocking of proteins". In: Smith BJ (ed.) Methods in Molecular Biology, vol. 64; Protein Sequencing Protocols, pp. 285–292. Totowa, NJ: Humana Press. | |
| Laursen R (1971) Solid-phase Edman degradation. An automatic peptide sequencer. European Journal of Biochemistry 20: 89–102. | |
| Sanger F (1959) Chemistry of insulin. Science 129: 1340–1344. | |
| Further Reading | |
| book Reim DF and Speicher DW (1995) "N-terminal analysis of proteins and peptides". In: Coligan JE, Dunn BM, Ploegh HL, Speicher DW and Wingfield PT (eds) Current Protocols in Protein Science, vol. 2, section 11.10. Chichester: Wiley. | |
| book Smith BJ (ed.) (1997) Protein Sequencing Protocols. Methods in Molecular Biology, vol. 64. Totowa, NJ: Humana Press. | |
| book Smith BJ and Chapman JR (1998) "Protein sequencing". In: Rapley R and Walker JM (eds) Molecular Biomethods Handbook, pp. 503–525. Totowa, NJ: Humana Press. | |
