Peptide Sequencing by Edman Degradation


Edman chemistry is a long‐established approach to determination of the primary structure, or sequence, of polypeptides.

Keywords: Edman chemistry; peptide sequence; protein sequence

Figure 1.

Edman chemistry for N‐terminal sequencing of polypeptides.



Edman P (1950) Method for determination of the amino acid sequence in peptides. Acta Chimica Scandinavica 4: 283–293.

Edman P and Begg G (1967) A protein sequenator. European Journal of Biochemistry 1: 80–91.

Hewick RM, Hunkapillar MW, Hood LE and Dreyer WJ (1981) A gas–liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry 256: 7990–7997.

Hirano H, Komatsu S and Tsunasawa S (1977) On membrane deblocking of proteins. In: Smith BJ (ed.) Methods in Molecular Biology, vol. 64; Protein Sequencing Protocols, pp. 285–292. Totowa, NJ: Humana Press.

Laursen R (1971) Solid‐phase Edman degradation. An automatic peptide sequencer. European Journal of Biochemistry 20: 89–102.

Sanger F (1959) Chemistry of insulin. Science 129: 1340–1344.

Further Reading

Reim DF and Speicher DW (1995) N‐terminal analysis of proteins and peptides. In: Coligan JE, Dunn BM, Ploegh HL, Speicher DW and Wingfield PT (eds) Current Protocols in Protein Science, vol. 2, section 11.10. Chichester: Wiley.

Smith BJ (ed.) (1997) Protein Sequencing Protocols. Methods in Molecular Biology, vol. 64. Totowa, NJ: Humana Press.

Smith BJ and Chapman JR (1998) Protein sequencing. In: Rapley R and Walker JM (eds) Molecular Biomethods Handbook, pp. 503–525. Totowa, NJ: Humana Press.

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How to Cite close
Smith, John Bryan(Apr 2001) Peptide Sequencing by Edman Degradation. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002688]