Transcription and Translation In Vitro

Martin J Tymms, National Vision Research Institute, Melbourne, Australia

Published online: April 2001

DOI: 10.1038/npg.els.0002691

In vitro transcription–translation provides the means to synthesize proteins rapidly from a DNA template sequence. Using polymerase chain reaction, DNA templates can be generated rapidly from any coding sequence and protein produced without the use of time-consuming protein expression systems using living cells.

Keywords: rabbit reticulocyte lysate; T7 polymerase; T7 promoter; protein truncation test

Figure 1. The different types of templates that can be used for in vitro transcription–translation.
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 References
    Kozak M (1994) Features in the 5¢ non-coding sequences of rabbit alpha and beta-globin mRNAs that affect translational efficiency. Journal of Molecular Biology 235: 95–110.
    Pelham HRB and Jackson RJ (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67: 247–256.
    Roest PA, Roberts RG, van der Tuijn AC et al. (1993) Protein truncation test (PTT) to rapidly screen the DMD gene for translation terminating mutations. Neuromuscular Disorders 3: 391–394.
 Further Reading
    book Tymms MJ (ed.) (1995) In Vitro Transcription and Translation Protocols. Totowa, New Jersey: Humana Press.

Related Sub-Topics:

Translation and Protein synthesis | Replication and Recombination | Transcription | Sample Preparation in Structural Biology | Transcription of DNA | Translation of RNA | Techniques and Tools in Molecular Biology | Proteins: Structure, Function, Metabolism | Enzymes: Structure and Action Mechanism | Nucleic Acids: Structure, Function, Metabolism
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Article Title: Transcription and Translation In Vitro
 
How to Cite close
Tymms, Martin J(Apr 2001) Transcription and Translation In Vitro. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002691]