Solid‐phase Peptide Synthesis: Fmoc


Synthetic peptides are increasingly important in biochemical, pharmacological, neurobiological, enzymological and molecular biological research. Use of Fmoc as temporary amino‐protecting group allows solid‐phase peptide synthesis with a milder acid cleavage process. Other advances in the ligation approach provide further potential for peptide synthesis.

Keywords: resin; loading; protecting group; cleavage; ligation; SPPS

Figure 1.

The scheme of the solid‐phase peptide synthesis. R: side chains; X: temporary α‐amino protecting group; Y: side chain protecting groups.



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Barany G, Albericio F, Biancalana S et al. (1992) Biopolymer syntheses on novel polyethylene glycol‐polystyrene (PEG‐PS) graft supports. In: Smith AJ and River JE (eds) Peptides: Chemistry and Biology Leiden: Escoml, pp. 603–604.

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Further Reading

Fields GB, Lauer‐Fields JL, Liu RQ and Barany G (2002) Principles and practice of solid‐phase peptide synthesis. In: Grant GA (ed.) Synthetic Peptides, A User's Guide, 2nd edn, pp. 93–219. New York: Oxford University Press.

Grant G (2002) Evaluation of the synthetic product. In Grant GA (ed.) Synthetic Peptides, A User's Guide, 2nd edn, pp. 220–291. New York: Oxford University Press.

Chan WC and White PD (2002) Fmoc Solid Phase Peptide Synthesis, A Practical Approach. New York: Oxford University Press.

Pennington MW and Dunn BM (1994) Peptide synthesis protocol. Methods in Molecular Biology 35. Totowa, NJ: Humana Press.

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Yang, Chi C(May 2005) Solid‐phase Peptide Synthesis: Fmoc. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002693]