Protein Synthesis: Measuring Errors


The detection and measurement of errors in protein synthesis is of interest to workers studying proteins, particularly when exploiting overexpression systems to prepare proteins as research reagents or for commercial purposes. Detection of errors is a key step in the process of producing high‐quality proteins.

Keywords: mistranslation; norleucine; norvaline; formylmethionine; ε‐N‐acetyllysine

Figure 1.

Structures of methionine, norleucine, leucine and norvaline.



Apostol I, Levine J, Lippincott J et al. (1997) Incorporation of norvaline at leucine positions in recombinant human hemoglobin expressed in Escherichia coli. Journal of Biological Chemistry 272: 28980–28988.

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Manley JL (1978) Synthesis and degradation of termination and premature‐termination fragments of β‐galactosidase in vitro and in vivo. Journal of Molecular Biology 125: 407–432.

Violand BN and Bogosian G (1998) The measurement of missense errors during in vivo protein synthesis. In: Martin R (ed.) Methods in Molecular Biology, vol. 77: Protein Synthesis: Methods and Protocols, pp. 143–151. Totowa, NJ: Humana Press, Inc.

Violand BN, Schlittler MR, Lawson CQ et al. (1994) Isolation of Escherichia coli synthesized recombinant eukaryotic proteins that contain ε‐N‐acetyllysine. Protein Science 3: 1089–1097.

Warren WC, Bentle KA, Schlittler MR, Schwane AC, O'Neil JP and Bogosian G (1996) Increased production of peptide deformylase eliminates retention of formylmethionine in bovine somatotropin overproduced in Escherichia coli. Gene 174: 235–238.

Further Reading

Parker J (1989) Errors and alternatives in reading the universal genetic code. Microbiological Reviews 53: 273–298.

Rosenberger RF and Holliday R (1993) Recombinant therapeutic proteins and translational errors. Trends in Biotechnology 11: 498–499.

Santos MAS and Tuite MF (1993) New insights into mRNA decoding – implications for heterologous protein synthesis. Trends in Biotechnology 11: 500–505.

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Bogosian, Gregg(Apr 2001) Protein Synthesis: Measuring Errors. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002694]