Protein Synthesis: Measuring Errors

Gregg Bogosian, Monsanto Company, Chesterfield, Missouri, USA

Published online: April 2001

DOI: 10.1038/npg.els.0002694

The detection and measurement of errors in protein synthesis is of interest to workers studying proteins, particularly when exploiting overexpression systems to prepare proteins as research reagents or for commercial purposes. Detection of errors is a key step in the process of producing high-quality proteins.

Keywords: mistranslation; norleucine; norvaline; formylmethionine; -N-acetyllysine

Figure 1. Structures of methionine, norleucine, leucine and norvaline.
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 References
    Apostol I, Levine J, Lippincott J et al. (1997) Incorporation of norvaline at leucine positions in recombinant human hemoglobin expressed in Escherichia coli. Journal of Biological Chemistry 272: 28980–28988.
    Bogosian G, Violand BN, Dorward-King EJ, Workman WE, Jung PE and Kane JF (1989) Biosynthesis and incorporation into protein of norleucine by Escherichia coli. Journal of Biological Chemistry 264: 531–539.
    book Bogosian G, Violand BN, Jung PE and Kane JF (1990) "Effect of protein overexpression on mistranslation in Escherichia coli". In: Hill WE, Dahlberg A, Garrett RS et al. (eds) The Ribosome: Structure, Function, and Evolution, pp. 546–558. Washington, DC: American Society for Microbiology.
    Kane JF, Violand BN, Curran DF, Staten NR, Duffin KL and Bogosian G (1992) Novel in-frame two codon translational hop during synthesis of bovine placental lactogen in a recombinant strain of Escherichia coli. Nucleic Acids Research 20: 6707–6712.
    Manley JL (1978) Synthesis and degradation of termination and premature-termination fragments of -galactosidase in vitro and in vivo. Journal of Molecular Biology 125: 407–432.
    book Violand BN and Bogosian G (1998) "The measurement of missense errors during in vivo protein synthesis". In: Martin R (ed.) Methods in Molecular Biology, vol. 77: Protein Synthesis: Methods and Protocols, pp. 143–151. Totowa, NJ: Humana Press, Inc.
    Violand BN, Schlittler MR, Lawson CQ et al. (1994) Isolation of Escherichia coli synthesized recombinant eukaryotic proteins that contain -N-acetyllysine. Protein Science 3: 1089–1097.
    Warren WC, Bentle KA, Schlittler MR, Schwane AC, O'Neil JP and Bogosian G (1996) Increased production of peptide deformylase eliminates retention of formylmethionine in bovine somatotropin overproduced in Escherichia coli. Gene 174: 235–238.
 Further Reading
    Parker J (1989) Errors and alternatives in reading the universal genetic code. Microbiological Reviews 53: 273–298.
    Rosenberger RF and Holliday R (1993) Recombinant therapeutic proteins and translational errors. Trends in Biotechnology 11: 498–499.
    Santos MAS and Tuite MF (1993) New insights into mRNA decoding – implications for heterologous protein synthesis. Trends in Biotechnology 11: 500–505.

Related Sub-Topics:

Protein Structure | Translation and Protein synthesis | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism
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Article Title: Protein Synthesis: Measuring Errors
 
How to Cite close
Bogosian, Gregg(Apr 2001) Protein Synthesis: Measuring Errors. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002694]