Alastair C Lewis, University of York, York, UK
Published online: May 2005
DOI: 10.1038/npg.els.0002705
Chromatography achieves the physical separation of components in a mixture for either analytical or preparative purposes, based on the unique degree of interaction between each component and the separating medium. Spanning a vast range of application areas, chromatography is probably one of the most commonly used laboratory techniques.
Keywords: liquid chromatography; gas chromatography; ion exchange chromatography; size exclusion chromatography; complex mixtures
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Figure 1. Schematic representation of the chromatographic process as it occurs in packed-column elution chromatography. Two components (marked as black and white spots) are introduced together as a mixture at the start of the column. As they pass through the packed bed of stationary phase particles, they are separated based on their interactions with the stationary phase. Above the column is the resulting chromatogram that would be obtained. The size/height of each peak is a function of the original analyte concentration and specific detector response to that compound.
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| Further Reading | |
| Chester TL and Parcher JF (2001) Blurring the boundaries. Science 291: 502–503. | |
| Lewis AC, Carslaw N, Marriott PJ et al. (2000) A larger pool of ozone-forming carbon compounds in urban atmospheres. Nature 405: 778–781. | |
| book McNair H and Miller J (eds) (1997) Basic Gas Chromatography Chichester: Wiley. | |
| book Meyer V (1999) Practical High-Performance Liquid Chromatography. Chichester: Wiley. | |
| Mohammad A, Fatima N, Ahmad J and Khan MAM (1993) Planar layer chromatography in the analysis of inorganic pollutants. Journal of Chromatography 642: 445–453. | |
| book Mondello L, Lewis AC and Bartle KD (eds) (2001) Multidimensional Chromatography. Chichester: Wiley. | |
| Poole CF (1999) Planar chromatography at the turn of the century. Journal of Chromatography A 856: 399–427. | |
| Poole CF and Dias NC (2000) Practitioner's guide to method development in thin-layer chromatography. Journal of Chromatography 892: 123–142. | |
| Poole SK, Kollie TO and Poole CF (1994) Influence of temperature on the mechanism by which compounds are retained in gas–liquid chromatography. Journal of Chromatography A 664: 229–251. | |
| Rudd PM, Guile GR, Kuster B et al. (1997) Oligosaccharide sequencing technology. Nature 388: 205–207. | |
| book Skoog DA, Holler FJ and Nieman TA (1998) Principles of Instrumental Analysis. Orlando, FL: Harcourt Brace. | |
| book Touchstone JC and Levin S (eds) (1990) Planar Chromatography in the Life Sciences. Chichester: Wiley. | |
| book Wu CS (ed) (1995) Handbook of Size Exclusion Chromatography. New York: Marcel Dekker. | |
| Zhou ZL, Licklider LJ, Gygi SP and Reed R (2002) Comprehensive proteomic analysis of the human spliceosome. Nature 419: 182–185. | |
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