Ghanshyam Swarup, Centre for Cellular and Molecular Biology, Hyderabad, India
Vegesna Radha, Centre for Cellular and Molecular Biology, Hyderabad, India
Published online: April 2001
DOI: 10.1038/npg.els.0002708
The nuclear matrix is the non-chromatin component of the nucleus, which consists of the nuclear envelope and the inner fibrous web forming a scaffold for attachment of DNA. Sequential extraction of isolated nuclei with DNAase and high-salt buffers yields a residual structure – the nuclear matrix.
Keywords: nuclear matrix; matrix isolation; nuclear architecture; nucleic acid metabolism; nuclear scaffold
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Figure 1. Steps involved in preparation of nuclear matrix.
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Figure 2. Polypeptide profile of various fractions obtained during isolation of nuclear matrix from nuclei of human HeLa cultured cells.
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| References | |
| Berezney R and Coffey DS (1974) Identification of a nuclear protein matrix. Biochemical and Biophysical Research Communications 60: 1410–1417. | |
| Hendzel MJ, Boisvert FM and Bazett-Jones DP (1999) Direct visualization of a protein nuclear architecture. Molecular Biology of the Cell 10: 2051–2062. | |
| Jackson DA and Cook PR (1988) Visualization of a filamentous nucleoskeleton with a 23 nm axial repeat. EMBO Journal 7: 3667–3677. | |
| Mancini MA, Shan B, Nickerson JA, Penman S and Lee W (1994) The retinoblastoma gene product is a cell cycle-dependent nuclear matrix-associated protein. Proceedings of the National Academy of Sciences of the USA 91: 418–422. | |
| Mirkovitch J, Mirrault ME and Laemmli UK (1984) Organization of the higher order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39: 223–232. | |
| Nickerson JA, Krockmalnic G, Wan KM and Penman S (1997) The nuclear matrix revealed by eluting chromatin from a cross-linked nucleus. Proceedings of the National Academy of Sciences of the USA 94: 4446–4450. | |
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| Further Reading | |
| book Berezney R and Jeon KW (eds) (1995) "Structural and functional organization of the nuclear matrix". International Review of Cytology 162A,B | |
| Hoffman M (1993) The cell's nucleus shapes up. Science 259: 1257–1259. | |
| Hozak P (1996) The nucleoskeleton and attached activities. Experimental Cell Research 229: 267–271. | |
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