Nuclear Matrix: Methods of Preparation

Abstract

The nuclear matrix is the non‐chromatin component of the nucleus, which consists of the nuclear envelope and the inner fibrous web forming a scaffold for attachment of DNA. Sequential extraction of isolated nuclei with DNAase and high‐salt buffers yields a residual structure – the nuclear matrix.

Keywords: nuclear matrix; matrix isolation; nuclear architecture; nucleic acid metabolism; nuclear scaffold

Figure 1.

Steps involved in preparation of nuclear matrix.

Figure 2.

Polypeptide profile of various fractions obtained during isolation of nuclear matrix from nuclei of human HeLa cultured cells.

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References

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Further Reading

Berezney R and Jeon KW (eds) (1995) Structural and functional organization of the nuclear matrix. International Review of Cytology 162A,B

Hoffman M (1993) The cell's nucleus shapes up. Science 259: 1257–1259.

Hozak P (1996) The nucleoskeleton and attached activities. Experimental Cell Research 229: 267–271.

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How to Cite close
Swarup, Ghanshyam, and Radha, Vegesna(Apr 2001) Nuclear Matrix: Methods of Preparation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002708]