Chromosomes: Methods for Preparation

Abstract

Visualization of the genome is possible within a short time frame during a cell division, the metaphase stage. Chromosomes can be arrested, fixed and used for the analysis.

Keywords: chromosome; preparation; diagnostics; genetic disease; cancer

Figure 1.

Human metaphase chromosomes (a) after fluorescence in situ hybridization (FISH) using the chromosome X‐specific centromeric probe labelled with spectrum orange and chromosome Y‐specific heterochromatin labelled with spectrum green; and (b) with the inverted DAPI (4′,6′‐diamino‐2‐phenylindole) banding (similar to G banding), allowing chromosomal identification.

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References

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Cremer T, Lichter P, Borden J, Ward DC and Manuelidis L (1988) Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome specific library probes. Human Genetics 80: 235–246.

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Further Reading

Bickmore WA (ed.) (1999) Chromosome Analysis: A Practical Approach. Oxford: Oxford University Press.

Pack SD and Zhuang Z (2000) Fluorescence in situ hybridization: application in cancer research and clinical diagnostics. Methods in Molecular Medicine 50: 35–50.

Rooney DE and Czepulkowski BH (1992) Human Cytogenetics, 2nd edn. New York: Oxford University Press.

Verma RS and Babu A (1995) Human Chromosomes. Principles and Techniques, 2nd edn. New York: McGraw‐Hill.

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How to Cite close
Pack, Svetlana D, and Stratakis, Constantine A(Mar 2002) Chromosomes: Methods for Preparation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002709]