Mass Spectrometry: Peptide Sequencing

Abstract

Electrospray and MALDI (matrix‐assisted laser desorption ionization) are two versions of desorption/ionization that allow modern mass spectrometry to be applied to the analysis of peptides and proteins. In addition to a direct indication of the molecular weight, the sequence of amino acids in a peptide can be deduced from the fragmentation pattern (the mass spectrum itself).

Keywords: electrospray; MALDI; signal suppression; mass spectrum; matrix‐assisted laser desorption ionization

Figure 1.

Conceptual respresentation of key bonds in a given peptide that break to form the indicated N‐terminal (a, b, c) or C‐terminal (x, y, z) fragments.

Figure 2.

ES‐CAD‐MS/MS mass spectrum of a peptide, LKRApTLG‐amide.

Figure 3.

ES‐CAD‐MS/MS mass spectrum of Ac‐TMPP derivative of LKRApTLG‐amide. The charged derivative (Ac‐TMPP) of the peptide promotes remote‐site fragmentation, which gives a more complete series of fragment ions (compared to that in Figure ) from which the sequence and locus of the phosphate group can be deduced.

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References

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Shen T‐L, Huang Z‐H, Laivenieks M et al. (1999) Evaluation of charge derivatization of a proteolytic digest for improved mass spectrometric analysis. Journal of Mass Spectrometry 34: 1154–1165.

Yates JR, Eng JK and McCormack AL (1995) Correlating MS/MS of peptides to sequences in nucleotide databases. Analytical Chemistry 67: 3202–3210.

Further Reading

Watson JT (1997) Introduction to Mass Spectrometry, 3rd edn. Philadelphia: Lippincott‐Raven.

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How to Cite close
Watson, J Throck(Apr 2001) Mass Spectrometry: Peptide Sequencing. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003111]