Capillary Blotting of RNA and DNA Gels


Quite often the gel electrophoretic migration pattern of individual components in a DNA or RNA sample is determined not by analysing the gel but rather by analysing an image of the gel created by blotting the gel on to membrane. One of the simplest procedures is capillary blotting.

Keywords: DNA transfer; Southern; blot; capillary

Figure 1.

Transfer apparatus.



Thurston SJ and Saffer JD (1989) Ultraviolet shadowing nucleic acids on nylon membranes. Analytical Biochemistry 178: 41–42.

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Southern, Edwin M(Jul 2003) Capillary Blotting of RNA and DNA Gels. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003745]