Capillary Blotting of RNA and DNA Gels

Edwin M Southern, University of Oxford, Oxford, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003745

Quite often the gel electrophoretic migration pattern of individual components in a DNA or RNA sample is determined not by analysing the gel but rather by analysing an image of the gel created by blotting the gel on to membrane. One of the simplest procedures is capillary blotting.

Keywords: DNA transfer; Southern; blot; capillary

Figure 1. Transfer apparatus.
close
 References
    Thurston SJ and Saffer JD (1989) Ultraviolet shadowing nucleic acids on nylon membranes. Analytical Biochemistry 178: 41–42.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Capillary Blotting of RNA and DNA Gels
 
How to Cite close
Southern, Edwin M(Jul 2003) Capillary Blotting of RNA and DNA Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003745]