Capillary Blotting of RNA and DNA Gels

Abstract

Quite often the gel electrophoretic migration pattern of individual components in a DNA or RNA sample is determined not by analysing the gel but rather by analysing an image of the gel created by blotting the gel on to membrane. One of the simplest procedures is capillary blotting.

Keywords: DNA transfer; Southern; blot; capillary

Figure 1.

Transfer apparatus.

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References

Thurston SJ and Saffer JD (1989) Ultraviolet shadowing nucleic acids on nylon membranes. Analytical Biochemistry 178: 41–42.

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How to Cite close
Southern, Edwin M(Jul 2003) Capillary Blotting of RNA and DNA Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003745]