Filamentous Phage Display

Donald B Palmer, The Royal Veterinary College, London, UK
Claudia De Lorenzo, University of Naples ‘Federico II’, Naples, Italy

Published online: January 2003

DOI: 10.1038/npg.els.0003752

Abstract

Bacteriophage display systems have been developed to allow the selection of a protein or peptide based on its ability to bind to a selected target molecule. The antibody‐display approach uses filamentous phage that express combinations of randomly assembled pairs of heavy and light chain genes either as a single chain antibody fragment or fragment antigen binding (Fab) format. These proteins are fused to the phage minor coat protein, pIII, to allow their expression and display on the surface of the phage, where they are available to bind antigen. Phage(s) expressing a particular sFv/Fab are enriched by panning on an immobilized target. Various antibody‐display phage libraries have been described and this system provides an alternative and powerful tool in generating specific antibodies.

Keywords: single chain antibody fragment; antibody phage display library; phagemid; helper phage

References

Clarkson T, Hoogenboom HR, Griffiths AD and Winter G (1991) Making antibody fragments using phage display libraries. Nature 352: 624–628.

Davies EL, Smith JS, Birkett CR et al. (1995) Selection of specific phage‐display antibodies using libraries derived from chicken immunoglobulin genes. Journal of Immunological Methods 186: 125–135.

de Kruif J, Boel E and Logtenberg T (1995a) Selection and application of human single chain Fv antibody fragments from a semi‐synthetic phage antibody display library with designed CDR3 regions. Journal of Molecular Biology 248: 97–105.

de Kruif J, Terstappen L, Boel E and Logtenberg T (1995b) Rapid selection of a cell subpopulation‐specific human monoclonal antibodies from a synthetic phage antibody library. Proceedings of the National Academy of Sciences of the USA 92: 3938–3942.

Griffiths AD, Williams SC, Hartley O et al. (1994) Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO Journal 13: 3245–3260.

Hawkins RE, Russell SJ and Winter G (1992) Selection of phage antibodies by binding affinity. Mimicking affinity maturation. Journal of Molecular Biology 226: 889–896.

Hoogenboom HR, Griffiths AD, Johnson KS et al. (1991) Multi‐subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Research 19: 4133–4137.

Hoogenboom HR, Marks JD, Griffiths AD and Winter G (1992) Building antibodies from their genes. Immunological Reviews 130: 41–68.

Johns M, George AJT and Ritter MA (2000) In vivo selection of sFv from phage display libraries. Journal of Immunological Methods 239: 137–151.

Lerner RA, Kang AS, Bain JD, Burton DR and Barbas CF III (1992) Antibodies without immunization. Science 258: 1313–1314.

McCafferty J, Griffiths AD, Winter G and Chiswell DJ (1990) Phage antibodies: filamentous phage display antibody variable domains. Nature 348: 552–554.

Marks JD, Hoogenboom HR, Bonnert TP et al. (1991) By‐passing immunization: human antibodies from V‐gene libraries displayed on phage. Journal of Molecular Biology 222: 581–597.

Nissim A, Hoogenboom HR, Tomlinson IM et al. (1994) Antibody fragments from a ‘single pot’ phage display library as immunochemical reagents. EMBO Journal 13: 692–698.

Orum H, Andersen PS, Oster A et al. (1993) Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage. Nucleic Acids Research 19: 4491–4498.

Palmer DB, George AJT and Ritter MA (1997) Selection of antibodies to cell surface determinants on mouse thymic epithelial cells using a phage display library. Immunology 91: 473–478.

Ridder R, Schmitz R, Legay F and Gram H (1995) Generation of rabbit monoclonal antibody fragment from a combinatorial phage display library and their production in the yeast Pichia pastoris. Biotechnology 13: 255–260.

Winter G, Griffiths AD, Hawkins RE and Hoogenboom HR (1994) Making antibodies by phage display technology. Annual Review of Immunology 12: 433–454.

Related Sub-Topics:

Antibodies | Virus Culture | Diagnostics, Therapeutics and Methods | Techniques and Tools in Molecular Biology
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Article Title: Filamentous Phage Display
 
How to Cite close
Palmer, Donald B, and Lorenzo, Claudia De(Jan 2003) Filamentous Phage Display. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003752]