Filamentous Phage Display
Donald B Palmer, The Royal Veterinary College, London, UK
Claudia De Lorenzo, University of Naples ‘Federico II’, Naples, Italy
Published online: January 2003
DOI: 10.1038/npg.els.0003752
Abstract
Bacteriophage display systems have been developed to allow the selection of a protein or peptide based on its ability to bind
to a selected target molecule. The antibody‐display approach uses filamentous phage that express combinations of randomly
assembled pairs of heavy and light chain genes either as a single chain antibody fragment or
fragment antigen binding (Fab) format. These proteins are fused to the phage minor coat protein, pIII, to allow their expression
and display on the surface of the phage, where they are available to bind antigen. Phage(s) expressing a particular sFv/Fab
are enriched by panning on an immobilized target. Various antibody‐display phage libraries have been described and this system
provides an alternative and powerful tool in generating specific antibodies.
Keywords: single chain antibody fragment; antibody phage display library; phagemid; helper phage
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