Protein Quantitation: Bradford Method

Abstract

Protein quantitation according to the Bradford method.

Keywords: protein quantitation; bradford; coomassie brilliant blue; calorimetric quantification

Figure 1.

Example of a calibration curve of bovine serum albumin according to the protocol of Bradford.

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References

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding. Analytical Biochemistry 72: 248–254.

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265–275.

Thorne CJR (1978) Techniques for determining protein concentration. In: Techniques in Protein and Enzyme Biochemistry B104, pp. 1–18. Amsterdam: Elsevier.

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How to Cite close
Gebauer, W(Oct 2002) Protein Quantitation: Bradford Method. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003753]