Protein Quantitation: Bradford Method

W Gebauer, University of Mainz, Mainz, Germany

Published online: October 2002

DOI: 10.1038/npg.els.0003753

Protein quantitation according to the Bradford method.

Keywords: protein quantitation; bradford; coomassie brilliant blue; calorimetric quantification

Figure 1. Example of a calibration curve of bovine serum albumin according to the protocol of Bradford.
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 References
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.
    Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265–275.
    book Thorne CJR (1978) "Techniques for determining protein concentration". In: Techniques in Protein and Enzyme Biochemistry B104, pp. 1–18. Amsterdam: Elsevier.

Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Techniques in Cell Biology | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism
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Article Title: Protein Quantitation: Bradford Method
 
How to Cite close
Gebauer, W(Oct 2002) Protein Quantitation: Bradford Method. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003753]