Protein Quantitation: Lowry Protocol


Protein quantitation according to the protocol of Lowry.

Keywords: protein quantitation; lowry; folin–ciocalteu; calorimetric quantification

Figure 1.

Example of a calibration curve of bovine serum albumin according to the protocol of Lowry.



Gornall AC, Bardawill CJ and David MM (1949) Determination of serum proteins by means of the Biuret reaction. Journal of Biological Chemistry 177: 751–766.

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265–275.

Thorne CJR (1978) Techniques for determining protein concentration. In: Techniques in Protein and Enzyme Biochemistry B104, pp. 1–18. Amsterdam: Elsevier.

Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

How to Cite close
Gebauer, W(Jul 2003) Protein Quantitation: Lowry Protocol. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003754]