Quantification of DNA and RNA: A Spectrophotometric Method

Bernhard Lieb, Johannes Gutenberg–University Mainz, Germany

Published online: July 2003

DOI: 10.1038/npg.els.0003755

Spectophotometric quantification is a very exact method of quantifying the content of nucleic acids in any sample. The purity of the sample can be simultaneously checked very easily.

Keywords: DNA; RNA; spectrophotometric quantification

 References
    book Alberts B, Watson JD, Roberts K et al. (1994) Molecular Biology of the Cell. New York: Garland Publishing.
    book Knippers R, Philippsen P, Schäfer KP and Fanning E (1990) Molekulare Genetik, 5th edn. Stuttgart: Thieme.
    book Qiagen Product Guide (1997) Appendices. Nucleic acids and proteins. Qiagen, Hilden, Germany.
    book Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
    book Stryer L (1995) Biochemistry, 4th edn. New York: Freeman.

Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Techniques in Cell Biology | Spectrophotometric Methods | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques | Nucleic Acids: Structure, Function, Metabolism
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Quantification of DNA and RNA: A Spectrophotometric Method
 
How to Cite close
Lieb, Bernhard(Jul 2003) Quantification of DNA and RNA: A Spectrophotometric Method. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003755]