Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters

Edwin M Southern, University of Oxford, Oxford, UK

Published online: January 2003

DOI: 10.1038/npg.els.0003757

One method of fixing DNA to filters prior to hybridization analysis is to use ultraviolet light to crosslink the DNA to the filter.

Keywords: DNA; UV fixation; nylon; filter

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters
 
How to Cite close
Southern, Edwin M(Jan 2003) Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003757]