Production of Polyclonal Antibodies in Rabbits


Polyclonal antibodies are produced by the immunization of laboratory rabbits with an immunogenic protein and the subsequent harvesting of antiserum. For smaller, less immunogenic proteins and peptides, conjugation of the molecule to a carrier protein is necessary before immunization.

Keywords: polyclonal antibodies; peptide conjugation; antiserum; immunization; adjuvant emulsion

Figure 1.

IgM antibodies predominate in the serum after the first injection, followed by high IgG production after the second injection.



Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.

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Liddell, JE(Jul 2003) Production of Polyclonal Antibodies in Rabbits. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003759]