Immunization of Mice for Monoclonal Antibody Production


Monoclonal antibody production requires the immunization of laboratory mice with an immunogenic protein and test sampling of antiserum. For smaller, less immunogenic proteins and peptides, conjugation of the molecule to a carrier protein is necessary before immunization.

Keywords: monoclonal antibodies; hybridomas; immunization; peptide conjugation; adjuvant emulsion

Figure 1.

IgM antibodies predominate in the serum after the first injection, followed by high IgG production after the second injection.



Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.

Liddell JE and Cryer A (1991) A Practical Guide to Monoclonal Antibodies. Chichester, UK: Wiley.

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Liddell, JE(Jul 2003) Immunization of Mice for Monoclonal Antibody Production. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003760]