Immunization of Mice for Monoclonal Antibody Production

JE Liddell, University of Cardiff, Cardiff, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003760

Monoclonal antibody production requires the immunization of laboratory mice with an immunogenic protein and test sampling of antiserum. For smaller, less immunogenic proteins and peptides, conjugation of the molecule to a carrier protein is necessary before immunization.

Keywords: monoclonal antibodies; hybridomas; immunization; peptide conjugation; adjuvant emulsion

Figure 1. IgM antibodies predominate in the serum after the first injection, followed by high IgG production after the second injection.
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 References
    book Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.
    book Liddell JE and Cryer A (1991) A Practical Guide to Monoclonal Antibodies. Chichester, UK: Wiley.

Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Antibodies | Techniques in Cell Biology
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Article Title: Immunization of Mice for Monoclonal Antibody Production
 
How to Cite close
Liddell, JE(Jul 2003) Immunization of Mice for Monoclonal Antibody Production. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003760]