Antibody Purification by Ammonium Sulfate Precipitation

JE Liddell, University of Cardiff, Cardiff, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003761

Ammonium sulfate precipitation of an antiserum or ascitic fluid will provide a crude immunoglobulin fraction free of other serum proteins. Purity is sufficient for the immunoglobulin to be used for labelling procedures or coating to an assay plate.

Keywords: antibody purification; ammonium sulfate; salt precipitation

 References
    book Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.

Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Antibodies | Techniques in Cell Biology | Biochemical and Biophysical Techniques
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Article Title: Antibody Purification by Ammonium Sulfate Precipitation
 
How to Cite close
Liddell, JE(Jul 2003) Antibody Purification by Ammonium Sulfate Precipitation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003761]