Antibody Purification by Ammonium Sulfate Precipitation

Abstract

Ammonium sulfate precipitation of an antiserum or ascitic fluid will provide a crude immunoglobulin fraction free of other serum proteins. Purity is sufficient for the immunoglobulin to be used for labelling procedures or coating to an assay plate.

Keywords: antibody purification; ammonium sulfate; salt precipitation

References

Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.

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How to Cite close
Liddell, JE(Jul 2003) Antibody Purification by Ammonium Sulfate Precipitation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003761]