Radioactive Labelling of Antibodies

JE Liddell, University of Cardiff, Cardiff, UK

Published online: March 2004

DOI: 10.1038/npg.els.0003762

Radioactive labelling of antibodies with 125Iodine (125I) by the chloramine T method is suitable for use in radioimmunoassays.

Keywords: radiolabelling; radioimmunoassays; chloramine T; 125iodine

 References
    Bolton AE and Hunter WM (1973) The labeling of proteins to high specific radioactivities by conjugation to an 125I-containing acylating agent. Biochemical Journal 133: 529–539.
    Fraker PJ and Speck JC Jr (1978) Protein and cell membrane iodinations with a sparingly soluble chloramide,1,3,4,6-tetrachloro-3a,6a-diphenyl glycouril. Biochemical and Biophysical Research Communications 80: 849–857.
    Greenwood FC, Hunter WM and Glover JS (1963) The preparation of 131I-labelled human growth hormone of high specific activity. Biochemical Journal 89: 114–123.
    Marchalonis JJ (1969) An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal 113: 229–305.

Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Antibodies | Techniques in Cell Biology
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Article Title: Radioactive Labelling of Antibodies
 
How to Cite close
Liddell, JE(Mar 2004) Radioactive Labelling of Antibodies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003762]