Radioactive Labelling of Antibodies

Abstract

Radioactive labelling of antibodies with 125Iodine (125I) by the chloramine T method is suitable for use in radioimmunoassays.

Keywords: radiolabelling; radioimmunoassays; chloramine T; 125iodine

References

Bolton AE and Hunter WM (1973) The labeling of proteins to high specific radioactivities by conjugation to an 125I‐containing acylating agent. Biochemical Journal 133: 529–539.

Fraker PJ and Speck JC Jr (1978) Protein and cell membrane iodinations with a sparingly soluble chloramide,1,3,4,6‐tetrachloro‐3a,6a‐diphenyl glycouril. Biochemical and Biophysical Research Communications 80: 849–857.

Greenwood FC, Hunter WM and Glover JS (1963) The preparation of 131I‐labelled human growth hormone of high specific activity. Biochemical Journal 89: 114–123.

Marchalonis JJ (1969) An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal 113: 229–305.

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How to Cite close
Liddell, JE(Mar 2004) Radioactive Labelling of Antibodies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003762]