Enzyme Labelling of Antibodies

Antibodies can be labelled with an enzyme such as horseradish peroxidase using the modified periodate method. A variety of different substrates are available giving soluble or insoluble products depending on the desired application.

Keywords: enzyme labelling; horseradish peroxidase; alkaline phosphate; -galactosidase; urease; glucose oxidase

 References
    Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6: 43–52.
    Avrameas S and Ternynck T (1971) Peroxidase labeled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8: 1175.
    Kato K, Hamaguchi Y, Fukui H and Ishikawa E (1975) Enzyme linked immunoassay II. A simple method for synthesis of the rabbit antibody--d-galactosidase complex and its general applicability. Journal of Biochemistry 78: 423–425.
    Kitagawa T and Aikawa T (1976) Enzyme coupled immunoassay of insulin using a novel coupling reagent. Journal of Biochemistry 79: 233–236.
    book Tijssen P (1988) "Practice and Theory of Enzyme Immunoassays". In: Burdon RH and van Knippenberg PH (eds) Laboratory Techniques in Biochemistry and Molecular Biology, vol. 15. Amsterdam: Elsevier.
    book Wilson MB and Nakane PK (1978) "Antibody conjugated to horseradish peroxidase". In: Knapp W, Holubar H and Wick G (eds) Immunofluorescence and Related Techniques, pp. 215–224. Amsterdam: Elsevier.
    Yoshitake S, Imagawa M and Ishikawa E et al. (1982) Mild and efficient conjugation of rabbit Fab and horseradish peroxidase using maleimide compound and its use for enzyme immunoassay. Journal of Biochemistry 92: 1413.
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Liddell, JE(Jan 2003) Enzyme Labelling of Antibodies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003763]