Enzyme‐linked Immunosorbent Assay (ELISA) to Measure Pure Protein

JE Liddell, School of Biosciences, University of Cardiff, Cardiff, UK

Published online: May 2003

DOI: 10.1038/npg.els.0003764

Enzyme-linked immunosorbent assay (ELISA) is used to measure the concentration of a soluble protein. The protein is coated to an assay plate and detected using a three-step detection process. A colour change is observed which is proportional to the concentration of protein bound to the plate.

Keywords: ELISA; immunoassay; protein measurement

Figure 1. ELISA to measure protein coated to the plate; indirect antibody label.
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 References
    book Kemeny DM (1991) A Practical Guide to ELISA. Oxford, UK: Pergamon Press.
    book Tijssen P (1988) "Practice and theory of enzyme immunoassays". In: Burdon RH and van Knippenberg PH (eds) Laboratory Techniques in Biochemistry and Molecular Biology, vol. 15. Amsterdam: Elsevier.

Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Antibodies | Techniques in Cell Biology | Biochemical and Biophysical Techniques
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Article Title: Enzyme‐linked Immunosorbent Assay (ELISA) to Measure Pure Protein
 
How to Cite close
Liddell, JE(May 2003) Enzyme‐linked Immunosorbent Assay (ELISA) to Measure Pure Protein. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003764]