Enzyme‐linked Immunosorbent Assay (ELISA) to Measure Pure Protein


Enzyme‐linked immunosorbent assay (ELISA) is used to measure the concentration of a soluble protein. The protein is coated to an assay plate and detected using a three‐step detection process. A colour change is observed which is proportional to the concentration of protein bound to the plate.

Keywords: ELISA; immunoassay; protein measurement

Figure 1.

ELISA to measure protein coated to the plate; indirect antibody label.



Kemeny DM (1991) A Practical Guide to ELISA. Oxford, UK: Pergamon Press.

Tijssen P (1988) Practice and theory of enzyme immunoassays. In: Burdon RH and van Knippenberg PH (eds) Laboratory Techniques in Biochemistry and Molecular Biology, vol. 15. Amsterdam: Elsevier.

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Liddell, JE(May 2003) Enzyme‐linked Immunosorbent Assay (ELISA) to Measure Pure Protein. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003764]