Conjugation of Proteins with Horseradish Peroxidase (HRP)

Tor Gjøen, University of Oslo, Oslo, Norway
Trond Berg, University of Oslo, Oslo, Norway

Published online: July 2003

DOI: 10.1038/npg.els.0003770

The intracellular pathway of endocytosed ligands can be followed by density shift methods. When the ligand is conjugated to an enzyme like horseradish peroxidase (HRP), the organelle can be made denser by the addition of a substrate for this enzyme, diaminobenzidine (DAB). When this substrate is metabolized in the presence of hydrogen peroxide (H2O2), an insoluble aggregate is formed and the organelle will increase in density.

Keywords: endocytosis; cell fractionation; peroxidase; density shift; lysosome; conjugation

 References
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Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Cell Compartments and Cellular Organelles | Techniques in Cell Biology | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism | Enzymes: Structure and Action Mechanism
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Article Title: Conjugation of Proteins with Horseradish Peroxidase (HRP)
 
How to Cite close
Gjøen, Tor, and Berg, Trond(Jul 2003) Conjugation of Proteins with Horseradish Peroxidase (HRP). In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003770]