Direct Labelling of Proteins with 125I

Tor Gjøen, University of Oslo, Oslo, Norway
Trond Berg, University of Oslo, Oslo, Norway

Published online: July 2003

DOI: 10.1038/npg.els.0003771

To quantify binding to receptors and endocytosis of protein ligands, radioactive labelling with 125I is still the most widely used technique. It combines high sensitivity with simple analysis and is the method of choice for most applications. Radioactive iodine can be directly coupled to tyrosine residues or via adducts that accumulate in the cells at their site of degradation.

Keywords: endocytosis; protein labelling; receptor; ligand; iodination; protein degradation

 References
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    Redshaw MR and Lynch SS (1974) An improved method for the preparation of iodinated antigens for radioimmunoassay. Journal of Endocrinology 60: 527–528.
    Steinman RM, Brodie SE and Cohn ZA (1976) Membrane flow during pinocytosis. A stereologic analysis. Journal of Cell Biology 68: 665–687.

Related Sub-Topics:

Protein Structure | Sample Preparation in Structural Biology | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism
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Article Title: Direct Labelling of Proteins with 125I
 
How to Cite close
Gjøen, Tor, and Berg, Trond(Jul 2003) Direct Labelling of Proteins with 125I. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003771]