Quantification of DNA and RNA: a Quantitative Visual Comparison

Abstract

The analytical procedure for the quantification of DNA and RNA described is the quantitative visual comparison of an unknown sample with known standards. This is quick and easy to do, suitable for amounts of sample as small as 10 ng.

Keywords: DNA; RNA; rapid quantification

Figure 1.

Schematic for estimation of the amount of DNA, by comparison with a known nucleic acid standard (e.g. 10 ng, 50 ng, 100 ng, 200 ng. Petri dish is filled with 1% agarose/ethidium bromide).

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References

Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

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How to Cite close
Lieb, Bernhard(Oct 2002) Quantification of DNA and RNA: a Quantitative Visual Comparison. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003772]