Quantification of DNA and RNA: a Quantitative Visual Comparison

Bernhard Lieb, Johannes Gutenberg‐University, Germany

Published online: October 2002

DOI: 10.1038/npg.els.0003772

The analytical procedure for the quantification of DNA and RNA described is the quantitative visual comparison of an unknown sample with known standards. This is quick and easy to do, suitable for amounts of sample as small as 10 ng.

Keywords: DNA; RNA; rapid quantification

Figure 1. Schematic for estimation of the amount of DNA, by comparison with a known nucleic acid standard (e.g. 10 ng, 50 ng, 100 ng, 200 ng. Petri dish is filled with 1% agarose/ethidium bromide).
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 References
    book Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Techniques in Cell Biology | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques | Nucleic Acids: Structure, Function, Metabolism
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Article Title: Quantification of DNA and RNA: a Quantitative Visual Comparison
 
How to Cite close
Lieb, Bernhard(Oct 2002) Quantification of DNA and RNA: a Quantitative Visual Comparison. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003772]