Affinity Purification of Antibodies


This method produces the purest sample of antibody, containing only antibodies that react with the desired antigen, free of all other immunoglobulins. It is based on affinity chromatography, in which antigen is bound to a solid phase to which specific antibody is bound and then eluted off.

Keywords: affinity chromatography; antibody purification; cyanogen‐bromide


Affinity Chromatography: Principles and Methods. Little Chalfont, UK: Amersham Pharmacia Biotech. []

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Liddell, JE(May 2003) Affinity Purification of Antibodies. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003774]