Immunofluorescence: Protocol


Immunocytochemistry is the only immunoassay in which antigen can be localized to its position in vivo in cells and tissues.

Keywords: FITC; TRITC; fluorescein isothiocyanate; immunocytochemistry

Figure 1.

Endothelial cells stained with a monoclonal anti‐von Willebrand factor antibody and a polyclonal rabbit anti‐von Willebrand factor antibody followed by (b) anti‐mouse IgG–FITC conjugate (green) and (a) anti‐rabbit IgG–TRITC conjugate (red). Note the more discrete staining of the monoclonal antibody since it only binds to one type of epitope.



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Liddell JE and Weeks I (1995) Antibody Technology. Oxford: BIOS Scientific Publishers.

Tijssen P (1988) Practice and Theory of Enzyme Immunoassays. Burdon RH and van Knippenberg PH (series eds) Laboratory Techniques in Biochemistry and Molecular Biology Series, vol 15. Amsterdam: Elsevier.

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Liddell, JE(Jul 2003) Immunofluorescence: Protocol. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003775]