Immunocytochemistry is the only immunoassay in which antigen can be localized to its position in vivo in cells and tissues.
Keywords: FITC; TRITC; fluorescein isothiocyanate; immunocytochemistry
Immunofluorescence: Protocol
JE Liddell, Cardiff School of Biosciences, Cardiff University, Cardiff, UK
Published online: July 2003
DOI: 10.1038/npg.els.0003775
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Figure 1. Endothelial cells stained with a monoclonal anti-von Willebrand factor antibody and a polyclonal rabbit anti-von Willebrand factor antibody followed by (b) anti-mouse IgG–FITC conjugate (green) and (a) anti-rabbit IgG–TRITC conjugate (red). Note the more discrete staining of the monoclonal antibody since it only binds to one type of epitope.
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| References | |
| book Beesley JE (ed.) (1993) Immunocytochemistry. A Practical Approach. Oxford: Oxford University Press. | |
| book Liddell JE and Weeks I (1995) Antibody Technology. Oxford: BIOS Scientific Publishers. | |
| book Tijssen P (1988) "Practice and Theory of Enzyme Immunoassays". Burdon RH and van Knippenberg PH (series eds) Laboratory Techniques in Biochemistry and Molecular Biology Series, vol 15. Amsterdam: Elsevier. | |
