Alkaline Blotting of DNA Gels

Abstract

Quite often the gel electrophoretic migration pattern of individual components in a sample is determined not by analysing the gel but rather by analysing an image of the gel created by blotting the gel on to membrane. One of the simplest procedures is capillary blotting. However, for blotting DNA gels using Zeta‚ÄźProbe, the blotting can be done in alkali, in which case the denatured DNA becomes fixed to the filter.

Keywords: southern; blot; alkaline; NaOH

Figure 1.

Transfer apparatus (cross‐section).

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References

Thurston SJ and Saffer JD (1989) Ultraviolet shadowing nucleic acids on nylon membrane. Analytical Biochemistry 178: 41.

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How to Cite close
Southern, Edwin M(Jul 2003) Alkaline Blotting of DNA Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003776]