Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes

Abstract

DNA and RNA are both readily stained by ethidium bromide. This method of detection is very sensitive and will detect very small amounts of sample. Once stained, the gels can be photographed to provide a permanent record.

Keywords: nucleic acid; gel; staining; fragment sizing

Figure 1.

UV illumination and photography apparatus: (a) incident light; (b) transillumination system.

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References

Brunk CF and Simpson L (1977) Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide–DNA complexes in polyacrylamide gels. Analytical Biochemistry 82: 455.

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How to Cite close
Southern, Edwin M(Jan 2003) Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003777]