Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes

Edwin M Southern, University of Oxford, Oxford, UK

Published online: January 2003

DOI: 10.1038/npg.els.0003777

DNA and RNA are both readily stained by ethidium bromide. This method of detection is very sensitive and will detect very small amounts of sample. Once stained, the gels can be photographed to provide a permanent record.

Keywords: nucleic acid; gel; staining; fragment sizing

Figure 1. UV illumination and photography apparatus: (a) incident light; (b) transillumination system.
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 References
    Brunk CF and Simpson L (1977) Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide–DNA complexes in polyacrylamide gels. Analytical Biochemistry 82: 455.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Statistical Methodology in Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes
 
How to Cite close
Southern, Edwin M(Jan 2003) Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003777]