Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA

Abstract

RNA and DNA can be separated under denaturing conditions using formamide polyacrylamide gels. Polyacrylamide gels are generally used for molecules and fragments between 6 bp and 1000 bp.

Keywords: PAGE

Figure 1.

Slab electrophoresis apparatus.

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References

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Fangman WL (1978) Separation of very large DNA molecules by gel electrophoresis. Nucleic Acids Research 5: 653.

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Jovin TM (1971) Electrophoretic fractionation of oligodeoxyribonucleotides. In: Grossman L and Moldave K (eds) Methods in Enzymology, vol. 21, p.179. London: Academic Press.

Maniatis T, Jeffrey A and van de Sande HV (1975) Chain length determination of small double‐ and single‐stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14: 3787.

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How to Cite close
Southern, Edwin M(May 2003) Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003779]