Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA

Edwin M Southern, University of Oxford, Oxford, UK

Published online: May 2003

DOI: 10.1038/npg.els.0003779

RNA and DNA can be separated under denaturing conditions using formamide polyacrylamide gels. Polyacrylamide gels are generally used for molecules and fragments between 6 bp and 1000 bp.

Keywords: PAGE

Figure 1. Slab electrophoresis apparatus.
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 References
    Bailey JM and Davidson N (1976) Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70: 75.
    Fangman WL (1978) Separation of very large DNA molecules by gel electrophoresis. Nucleic Acids Research 5: 653.
    Gould HJ and Hamlyn PH (1973) The molecular weight of rabbit globin messenger RNAs. FEBS Letters 30: 301.
    book Jovin TM (1971) "Electrophoretic fractionation of oligodeoxyribonucleotides". In: Grossman L and Moldave K (eds) Methods in Enzymology, vol. 21, p.179. London: Academic Press.
    Maniatis T, Jeffrey A and van de Sande HV (1975) Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14: 3787.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
 
How to Cite close
Southern, Edwin M(May 2003) Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003779]