Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels

Abstract

Formaldehyde reacts with RNA, and when RNA is heated in the presence of formaldehyde all secondary structures are removed. Hence, gel electrophoresis of RNA in agarose gels containing formaldehyde provides a good denaturing gel system.

Keywords: denaturing; electrophoresis; RNA; agarose; formaldehyde

Figure 1.

Submarine slab gel apparatus.

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References

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Maniatis T, Jeffrey A and van de Sande HV (1975) Chain length determination of small double‐ and single‐stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14: 3787.

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Southern, Edwin M(Jul 2003) Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003780]