Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels


Formaldehyde reacts with RNA, and when RNA is heated in the presence of formaldehyde all secondary structures are removed. Hence, gel electrophoresis of RNA in agarose gels containing formaldehyde provides a good denaturing gel system.

Keywords: denaturing; electrophoresis; RNA; agarose; formaldehyde

Figure 1.

Submarine slab gel apparatus.



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Southern, Edwin M(Jul 2003) Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0003780]