Isolation of DNA from Agarose Gels using Agarase

Edwin M Southern, University of Oxford, Oxford, UK

Published online: March 2003

DOI: 10.1038/npg.els.0003788

One of the major advantages of low gelling temperature agarose is that it is easy to recover DNA and RNA from the gel by melting it and extracting it with phenol. This basic procedure may be improved by including an agarase digestion step.

Keywords: DNA; isolation; agarase

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Isolation of DNA from Agarose Gels using Agarase
 
How to Cite close
Southern, Edwin M(Mar 2003) Isolation of DNA from Agarose Gels using Agarase. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003788]