Denaturation of RNA Using Glyoxal

Edwin M Southern, University of Oxford, Oxford, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003789

RNA normally exhibits a high degree of secondary structure and aggregation. By treating RNA with glyoxal the RNA molecules can be completely denatured and assume random coil configurations.

Keywords: RNA; denaturation; glyoxal; electrophoresis

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Denaturation of RNA Using Glyoxal
 
How to Cite close
Southern, Edwin M(Jul 2003) Denaturation of RNA Using Glyoxal. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003789]