Denaturation of RNA Using Glyoxal

Abstract

RNA normally exhibits a high degree of secondary structure and aggregation. By treating RNA with glyoxal the RNA molecules can be completely denatured and assume random coil configurations.

Keywords: RNA; denaturation; glyoxal; electrophoresis

Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

How to Cite close
Southern, Edwin M(Jul 2003) Denaturation of RNA Using Glyoxal. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003789]