Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis

Edwin M Southern, University of Oxford, Oxford, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003792

Chromosomal DNA of many eukaryotes is too large to be separated by pulsed field gel electrophoresis. DNA can be digested in agarose plugs for pulsed field gel electrophoresis.

Keywords: DNA; digest; agarose; pulsed field gel electrophoresis

 References
    Bird AP (1986) CpG-rich islands and the function of DNA methylation. Nature 321: 209.
    Tyler-Smith C (1987) Structure of repeated sequences in the centromeric region of the human Y chromosome. Development 101 (suppl.): 93.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
 
How to Cite close
Southern, Edwin M(Jul 2003) Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003792]