Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis

Abstract

Chromosomal DNA of many eukaryotes is too large to be separated by pulsed field gel electrophoresis. DNA can be digested in agarose plugs for pulsed field gel electrophoresis.

Keywords: DNA; digest; agarose; pulsed field gel electrophoresis

References

Bird AP (1986) CpG‐rich islands and the function of DNA methylation. Nature 321: 209.

Tyler‐Smith C (1987) Structure of repeated sequences in the centromeric region of the human Y chromosome. Development 101 (suppl.): 93.

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How to Cite close
Southern, Edwin M(Jul 2003) Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003792]