Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming

S Sheardown, MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003793

The ability to label nucleic acids is one of the most fundamental techniques in molecular biology. Radiolabelling is still the method of choice for the most sensitive applications and also in situations where it would be difficult to visualize a nonradioactive label, such as the gel mobility shift assay where the probe remains within the gel matrix.

Keywords: 32P

 References
    Feinberg AP and Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132: 6–13.
    Feinberg AP and Vogelstein B (1984) Addendum: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137: 266–267.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
 
How to Cite close
Sheardown, S(Jul 2003) Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003793]