Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming

Abstract

The ability to label nucleic acids is one of the most fundamental techniques in molecular biology. Radiolabelling is still the method of choice for the most sensitive applications and also in situations where it would be difficult to visualize a nonradioactive label, such as the gel mobility shift assay where the probe remains within the gel matrix.

Keywords: 32P

References

Feinberg AP and Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132: 6–13.

Feinberg AP and Vogelstein B (1984) Addendum: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137: 266–267.

Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

How to Cite close
Sheardown, S(Jul 2003) Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003793]