Manual Dot Blotting

Abstract

DNA blots can be prepared without specialized apparatus, though the results are less good than those obtained by manifold blotting. However, the technique may be useful for ‘in‐house’ purposes such as roughly comparing the levels of nonradioactive labelling of probes. Only very small volumes of sample can be used.

Keywords: DNA; membrane; denaturation; neutralization; immobilization

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How to Cite close
Beauchamp, J(Jul 2003) Manual Dot Blotting. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003796]