Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane

Abstract

Manifold dot or slot blotting of DNA on to nitrocellulose or uncharged nylon membranes are described together as they involve the same steps but differ in the buffers used and in the method of post‐blotting fixation.

Keywords: denaturation; neutralization; immobilization

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How to Cite close
Beauchamp, J(Jul 2003) Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003797]