Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane

J Beauchamp, Imperial College School of Medicine, London, UK

Published online: July 2003

DOI: 10.1038/npg.els.0003797

Manifold dot or slot blotting of DNA on to nitrocellulose or uncharged nylon membranes are described together as they involve the same steps but differ in the buffers used and in the method of post-blotting fixation.

Keywords: denaturation; neutralization; immobilization

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
 
How to Cite close
Beauchamp, J(Jul 2003) Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003797]