Site-directed mutagenesis can be used to mutate DNA fragments inserted into plasmid vectors using one oligonucleotide and three restriction enzymes.
Keywords: mutagens
Site‐directed Mutagenesis of Double‐stranded Plasmids
P Jones, Zeneca Agrochemicals, Bracknell, UK
Published online: July 2003
DOI: 10.1038/npg.els.0003798
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Figure 1. Site-directed mutagenesis of double-stranded plasmids.
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| References | |
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