Single‐step Method of Total RNA Isolation by Guanidine–Phenol Extraction

Piotr Chomczynski, Molecular Research Center Inc., Cincinnati, Ohio, USA

Published online: May 2005

DOI: 10.1038/npg.els.0003799

The single-step method isolates total RNA from a variety of sources including tissues and cells from human, animal, plant, yeast, bacterial and viral origins. The method is based on liquid-phase separation resulting in sequestration of pure RNA into the aqueous phase. RNA is precipitated from the aqueous phase, dissolved, reprecipitated and washed with alcohol. The entire procedure can be completed in less than 4 h.

Keywords: ribonucleic acid; single-step method; liquid-phase separation; total RNA isolation

 References
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Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Single‐step Method of Total RNA Isolation by Guanidine–Phenol Extraction
 
How to Cite close
Chomczynski, Piotr(May 2005) Single‐step Method of Total RNA Isolation by Guanidine–Phenol Extraction. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003799]