Isolation of Plasmid DNA from E. coli

P Jones, Zeneca Agrochemicals, Bracknell, UK

Published online: May 2003

DOI: 10.1038/npg.els.0003800

The use of plasmid DNA is the keystone of DNA analysis because it allows easy manipulation and maintenance of defined heterologous DNA fragments. The Escherichia coli bacterial system is very versatile, allowing rapid DNA replication and informed gene manipulation.

Keywords: XL2-blue; stratagene

Related Sub-Topics:

Techniques in Cell Biology | Techniques and Tools in Molecular Biology | Nonchromosomal DNA | Bacteria | Genomes and Chromosomes
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Article Title: Isolation of Plasmid DNA from E. coli
 
How to Cite close
Jones, P(May 2003) Isolation of Plasmid DNA from E. coli. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003800]