Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media

John M Graham, Wirral, UK

Published online: March 2004

DOI: 10.1038/npg.els.0003802

Rat liver and other soft tissue from small mammals can be homogenized using a Potter-Elvehjem (Teflon/glass) homogenizer.

Keywords: rat; liver; homogenization; nuclei; mitochondria; peroxisomes; organelles

 References
    book Evans WH (1992) "Isolation and characterization of membranes and cell organelles". In: Rickwood D (ed.) Preparative Centrifugation – a Practical Approach, pp. 233–270. Oxford: Oxford University Press.
    book Graham JM (1997) "Homogenization of tissues and cells". In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 1–29. Oxford: Oxford University Press.
    Marsh M, Schmid S, Kern H et al. (1987) Rapid analytical and preparative isolation of functional endosomes by free flow electrophoresis. Journal of Cell Biology 104: 875–886.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media
 
How to Cite close
Graham, John M(Mar 2004) Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003802]