Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media

Abstract

Rat liver and other soft tissue from small mammals can be homogenized using a Potter‐Elvehjem (Teflon/glass) homogenizer.

Keywords: rat; liver; homogenization; nuclei; mitochondria; peroxisomes; organelles

References

Evans WH (1992) Isolation and characterization of membranes and cell organelles. In: Rickwood D (ed.) Preparative Centrifugation – a Practical Approach, pp. 233–270. Oxford: Oxford University Press.

Graham JM (1997) Homogenization of tissues and cells. In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 1–29. Oxford: Oxford University Press.

Marsh M, Schmid S, Kern H et al. (1987) Rapid analytical and preparative isolation of functional endosomes by free flow electrophoresis. Journal of Cell Biology 104: 875–886.

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How to Cite close
Graham, John M(Mar 2004) Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003802]